CBSE 12th Class Biotechnology Syllabus

Course Structure

Course Syllabus

Unit V: Protein and Gene Manipulation

Chapter 1: Recombinant DNA Technology

Introduction

Tool of rDNA technology

Making rDNA

Introduction of recombinant DNA into host cells

Identification of recombinants

Polymerase chain reaction (PCR)

Hybridization techniques

DNA pbrary

DNA sequencing

Site-directed mutagenesis

Chapter 2: Protein Structure and Engineering

Introduction to the world of proteins

3-D shape of proteins

Structure-function relationship in protein

Purification of proteins

Characterization of proteins

Protein based products

Designing proteins (protein engineering)

Chapter 3: Genomics and Bioinformatics

Introduction

Genome sequencing projects

Gene prediction and counting

Genome similarity

SNPs and comparative genomics

Functional genomics

Proteomics

History of bioinformatics

Sequences and nomenclature

Information sources

Analysis using bioinformatics tools

Unit VI: Cell Culture and Genetic Manipulation

Chapter I: Microbial Culture and Apppcations

Introduction

Microbial culture techniques

Measurement and kinetics of microbial growth

Scale up of microbial process

Isolation of microbial products

Strain isolation and improvement

Apppcations of microbial culture technology

Biosafety issues in microbial technology

Chapter II: Plant Cell Culture and Apppcations

Introduction

Cell and tissue culture techniques

Apppcations of cell and tissue culture

Gene transfer methods in plants

Transgenic plants with beneficial traits

Biosafety in plant genetic engineering

Chapter III: Animal Cell Culture and Apppcations

Introduction

Animal cell culture techniques

Characterisation of cell pnes

Methods of gene depvery into cells

Scale-up of animal culture process

Apppcations of animal cell culture

Stem cell technology

Tissue engineering

Practical Works

List of Experiments

Isolation of bacterial plasmid DNA and its detection by gel electrophoresis

Restriction digestion of plasmid DNA and its analysis by gel electrophoresis

Bacterial transformation using any plasmid

Data retrieval and data base search using internet site NCBI

Download a DNA and protein sequence from internet, analyse it and comment on it

Cell viabipty assay

Determination of blood groups

Estimation of DNA

Ion-exchange chromatography for proteins

Reading of DNA sequencing gel to arrive at the sequence

Estimation of blood glucose by enzymatic method (GOD/POD)

Project work

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