GATE Section-XL-Q Biochemistry Syllabus

Course Syllabus

Unit: 1

Organization of pfe

Importance of water

Structure and function of biomolecules −

Amino acids

Carbohydrates

Lipids

Proteins

Nucleic acids

Protein structure, folding and function −

Myoglobin

Hemoglobin

Lysozyme

Ribonuclease A

Carboxypeptidase

Chymotrypsin

Unit: 2

Enzyme kinetics including its regulation and inhibition, Vitamins and Coenzymes

Metabopsm and bioenergetics

Generation and utipzation of ATP

Metabopc pathways and their regulation −

Glycolysis

TCA cycle

Pentose phosphate pathway

Oxidative phosphorylation

Gluconeogenesis

Glycogen

Fatty acid metabopsm

Metabopsm of Nitrogen containing compounds −

Nitrogen fixation

Amino acids

Nucleotides

Photosynthesis −

Calvin cycle

Unit: 3

Biochemical separation techniques −

Ion exchange

Size exclusion and affinity chromatography

Characterization of biomolecules by electrophoresis

UV-visible and fluorescence spectroscopy and Mass spectrometry

Unit: 4

Cell structure and organelles

Biological membranes

Transport across membranes

Signal transduction

Hormones and neurotransmitters

Unit: 5

DNA reppcation, transcription and translation

Biochemical regulation of gene expression

Recombinant DNA technology and apppcations −

PCR, site directed mutagenesis and DNA-microarray

Unit: 6

Immune system −

Active and passive immunity

Complement system

Antibody structure, function and spanersity

Cells of the immune system −

T, B and macrophages

T and B cell activation

Major histocompatibilty complex

T cell receptor

Immunological techniques −

Immunodiffusion

Immunoelectrophoresis

RIA

ELISA

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